FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5175670
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18650.049.8318563
CCGAGTA20450.048.969072
TCGCGTT2200.042.5437435
CCCGAGT28200.040.9927751
CGGAGGT19850.038.809527
GAGTAGC47350.037.7157024
CTCGCGT2600.036.004814
AGTAGCT74100.035.949975
GTAGCTG74050.035.8770146
GCTGGGA132800.035.0226829
AGGCGGA29850.034.9795464
GGCGGAG31300.034.0433545
CGCGTTG2750.034.0349926
GGAGGTT56550.033.9933248
TTGGGAG109250.033.280126
TAGCTGG83900.031.8366187
GGGAGGC134100.029.9048338
GAGGTTG73000.029.8837289
GCGGAGG27900.029.6762246
GGAGGCT105700.029.2892639
Produced by FastQC (version 0.11.5)