FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5697275
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21350.048.223582
CGAGTAG21050.047.200683
AGTAGCT85850.043.274365
GAGTAGC52950.042.695784
TTGGGAG128300.042.256126
CGGAGGT28300.041.9777157
GCTGGGA168750.040.8736089
CTACTCG21650.039.906592
GTAGCTG91500.039.5793046
CCCGAGT29550.039.232921
TAGCTGG98900.038.7290277
TACTCGG22750.038.6099933
GGAGGCT140300.038.3340039
GGGAGGC171050.037.2092138
GGAGGTT74650.035.7820638
GCTACTC55000.035.4804271
TGGGAGG171900.033.967717
AGGCGGA42800.032.971114
TCGGGAG28700.032.6123966
GCACTTT121900.032.3712231
Produced by FastQC (version 0.11.5)