FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5043795
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG20050.033.037182
TACTCGG21350.031.7000183
AGTAGCT79000.028.2529745
GCTGGGA157750.028.1151819
GAGTAGC51300.027.2278864
CGAGTAG22450.026.6189753
TAGCTGG91950.026.4663777
GTAGCTG83400.026.2444256
CCGAGTA23550.025.9870832
GCTACTC50850.025.6304321
TTGGGAG112450.024.3306476
TCGGGAG30250.024.0395076
CTCGGGA28500.023.4945775
GGAGGCT130400.023.1900259
ACTCGGG31400.023.1590794
GGGAGGC155600.022.0255588
CCCAAAG127100.021.0182531
AAGTGCT102100.021.0145475
CCAAAGT115400.020.963462
GTGCTGG105500.020.7467787
Produced by FastQC (version 0.11.5)