FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6599909
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24700.049.8450623
CCGAGTA27000.048.5322722
CGGAGGT33000.047.126247
TACTCGG26200.043.6937033
GAGTAGC63000.043.427624
AGTAGCT99600.043.155685
GCTGGGA195950.041.887329
TTGGGAG154850.041.334586
CTACTCG26900.041.218432
GTAGCTG107650.040.39676
GGAGGTT84900.040.1978
GCTACTC64600.039.5692981
CCCGAGT37850.039.1889651
TAGCTGG115800.038.424037
GGAGGCT157750.037.8362549
GGGAGGC193800.037.6718
CTCGGGA30050.036.8976945
AGGCGGA48700.036.2209744
GGCGGAG50100.035.9273575
TCGGGAG32450.035.278126
Produced by FastQC (version 0.11.5)