FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5572461
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28750.063.872713
GAGTAGC68550.062.924074
AGTAGCT111150.062.1849255
CCGAGTA31050.061.2375342
GTAGCTG120850.057.074516
GCTGGGA235200.054.2437639
TTGGGAG171600.052.907986
TAGCTGG138100.051.300887
CCCGAGT39850.049.7799721
CTACTCG32950.047.6514852
TACTCGG32850.047.5704653
GGAGGCT187300.046.820169
TCGGGAG37400.046.394756
TTTGGGA164950.045.8747565
GGGAGGC241450.044.6095478
CGGAGGT42500.044.0461357
GCACTTT149700.044.0427741
AAGTGCT142350.043.2446145
GCTACTC78750.042.5944181
CTCGGGA37100.042.5004545
Produced by FastQC (version 0.11.5)