FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5732309
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26200.065.9524843
CCGAGTA27150.065.766252
GAGTAGC62450.063.0632134
AGTAGCT105550.060.367925
GTAGCTG116500.054.0140466
GCTGGGA234950.052.8915869
TTGGGAG172100.052.0846446
CCCGAGT38700.050.982091
TAGCTGG136250.048.615257
TACTCGG30950.047.6885833
GGAGGCT176800.046.2612429
CTACTCG32100.045.082942
TTTGGGA169300.044.483195
CGGAGGT40350.044.2516377
GCACTTT147900.043.7206081
GGGAGGC229450.043.3024258
GCTACTC77350.043.2883151
AAGTGCT140700.042.676935
ACTTTGG157450.041.2463073
CAAGTAG52900.040.8306963
Produced by FastQC (version 0.11.5)