FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107276
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27600.070.173493
AGTAGCT104350.066.927545
GAGTAGC65600.065.633114
CCGAGTA30100.062.670722
TTGGGAG166200.062.5116546
GTAGCTG114800.060.647136
GCTGGGA227600.057.6370549
GCACTTT141000.056.1786771
CCCGAGT36100.055.254861
TAGCTGG130400.053.9439357
TTTGGGA160200.052.9429865
ACTTTGG148000.052.540233
GGAGGCT190200.051.6332559
CACTTTG152700.050.4515652
GGGAGGC248950.049.5416958
TACTCGG34600.048.4852563
CTACTCG33350.048.3595282
AAGTGCT140950.048.1695145
TGGGAGG234800.047.1304327
CTTTGGG174300.046.5534824
Produced by FastQC (version 0.11.5)