FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4567876
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27500.075.1515663
CCGAGTA28950.071.647222
AGTAGCT108700.069.348875
GAGTAGC69150.068.93664
GTAGCTG114450.065.046966
GCTGGGA234600.057.7889189
TAGCTGG134550.057.2026637
TTGGGAG159100.056.702626
CCCGAGT39200.054.850481
CCAAGTA51550.050.4352232
GGAGGCT177800.049.9695749
GCACTTT129700.049.6220971
TTTGGGA149800.049.3606535
CAAGTAG49500.049.3154873
TGAGTAG56550.047.3695373
AAGTGCT128750.047.029575
ACTTTGG137650.046.8727723
CTACTCG33250.046.569572
CTGAGTA57200.045.9570582
GCTACTC74000.045.9239731
Produced by FastQC (version 0.11.5)