FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5009174
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25400.071.149383
CCGAGTA26200.070.625742
AGTAGCT104200.068.959485
GAGTAGC65150.067.523974
GTAGCTG110900.063.2351536
GCTGGGA224550.056.6571439
CCCGAGT34450.056.2294161
TTGGGAG157650.055.4898386
TAGCTGG130650.054.8884667
CTACTCG29150.051.6224822
TACTCGG29450.051.585583
TCGGGAG34200.049.263016
GGAGGCT174850.049.248929
TTTGGGA150700.046.916915
CAAGTAG51900.046.8900343
GCACTTT130700.046.3913571
GGGAGGC227550.046.3545078
AAGTGCT134700.045.3807075
AAGTAGC56250.044.9278644
GCTACTC73350.044.7677461
Produced by FastQC (version 0.11.5)