FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297979
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA28200.061.786212
CGAGTAG28100.061.7498663
AGTAGCT103950.057.8345345
GAGTAGC66000.056.7263264
GTAGCTG111350.053.667726
TTGGGAG158050.051.2943086
CCCGAGT37050.049.949561
GCTGGGA224300.049.7860649
TAGCTGG128300.048.541677
GCACTTT131650.045.3440631
TTTGGGA153250.043.786555
ACTTTGG140900.042.5656553
CTACTCG30150.042.029152
TACTCGG31250.041.9321023
GGAGGCT172250.041.924419
GGGAGGC227300.041.653518
CACTTTG143200.041.2786452
AAGTGCT129850.040.255015
CTTTGGG163650.038.6721344
CAAGTAG52900.038.245113
Produced by FastQC (version 0.11.5)