FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4903878
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG36750.084.2511143
AGTAGCT136500.083.0226755
CCGAGTA38600.080.0380552
GAGTAGC89100.078.632064
GTAGCTG145100.077.1095666
TAGCTGG171250.066.554127
GCTGGGA301550.066.137099
CAAGTAG59100.065.3043063
CCCGAGT49550.064.771741
CCAAGTA63100.062.0863342
TTGGGAG186650.061.6415376
AAGTAGC66550.060.0495034
TGAGTAG64300.057.4474683
CTGAGTA67500.055.6920552
TTTGGGA171400.054.5241055
AAGTGCT160800.054.22295
GGAGGCT214450.053.6171239
GCACTTT149250.053.590331
CTACTCG41150.051.9772262
ACTTTGG157600.051.4911043
Produced by FastQC (version 0.11.5)