FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4903878
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG34050.087.326663
AGTAGCT130850.083.193995
GAGTAGC83150.081.911114
CCGAGTA37250.079.631492
GTAGCTG138600.077.86686
GCTGGGA295900.066.911649
TTGGGAG203550.066.35536
TAGCTGG166150.066.255377
CCCGAGT46800.062.310741
CCAAGTA59700.060.7813222
GCACTTT161500.060.634551
CAAGTAG58200.060.4922563
TGAGTAG63100.059.9023553
AAGTGCT155250.059.7771575
TTTGGGA185800.059.7132725
ACTTTGG171400.058.345233
CACTTTG173850.056.529072
AAGTAGC63950.056.516744
GGAGGCT234300.056.386839
CTGAGTA67600.056.340792
Produced by FastQC (version 0.11.5)