FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4243823
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT114000.085.6405265
CGAGTAG30900.084.347963
CCGAGTA31600.083.163032
GAGTAGC74550.081.414994
GTAGCTG124600.077.719286
TTGGGAG177800.068.232716
GCTGGGA263200.066.2267469
TAGCTGG150700.066.0744257
CCCGAGT41800.065.804031
CAAGTAG49600.064.4504243
CCAAGTA51350.064.216932
GCACTTT139150.062.095981
TTTGGGA160450.060.7585
ACTTTGG145800.060.097663
AAGTAGC55300.059.630014
CACTTTG147250.058.3323752
GGAGGCT198200.058.1946879
CTGAGTA56450.057.0122382
TGAGTAG57850.056.628173
AAGTGCT137450.056.362735
Produced by FastQC (version 0.11.5)