FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5272433
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA32350.068.103692
CGAGTAG31700.067.456023
AGTAGCT113750.067.219085
GAGTAGC70450.066.01934
GTAGCTG120400.063.0878036
GCTGGGA241450.057.8487439
TAGCTGG140450.055.7220657
CCCGAGT41100.054.6625441
TTGGGAG166400.054.1714486
GGAGGCT192150.047.923629
GCACTTT142450.046.40421
CTACTCG33900.046.0875472
AAGTGCT144750.045.511585
TACTCGG35300.045.4834823
CAAGTAG55600.044.675343
ACTTTGG146750.044.646443
TTTGGGA162400.044.5996445
GGGAGGC253350.043.3663678
AAGTAGC60200.043.174784
CACTTTG153050.042.9497832
Produced by FastQC (version 0.11.5)