FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325456
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA27900.029.441272
AGTAGCT111200.028.2936615
GAGTAGC70300.027.2482364
CGAGTAG27200.027.0201153
GTAGCTG114350.026.94766
GCTGGGA216250.025.9021599
TAGCTGG133050.024.9458627
TTGGGAG149650.024.9210016
CCCGAGT41600.022.237021
CTACTCG30800.021.5225142
TCGCGTT2353.9181545E-521.445825
GCTACTC73850.021.0401061
GGGAGGC207650.020.5952458
TACTCGG30850.020.5533873
GGAGGCT172000.020.0083799
AAGTAGC59900.019.5962434
GCACTTT132800.019.536931
CCCAAGT74800.019.4202331
TGGGAGG210250.019.4159897
AAGTGCT138200.019.2755225
Produced by FastQC (version 0.11.5)