FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325456
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA31200.035.547832
CGAGTAG31250.034.3283353
GAGTAGC72500.030.4871484
AGTAGCT114950.029.938875
GTAGCTG118850.028.9564426
GCTGGGA218950.028.1149719
TAGCTGG137200.027.3400977
CCCGAGT46100.026.8997731
CTACTCG27900.026.329522
TACTCGG28800.026.2490333
TTGGGAG145700.023.4720236
TCGCGTT3254.3040927E-822.1530285
GCTACTC72500.021.7784611
CAAGTAG55400.020.403583
AGCTGGG195250.020.3915658
TCGGGAG39550.020.388636
CTCGGGA36850.020.1240885
GGAGGCT174150.020.0922819
GGGAGGC207100.020.024378
CCCAAAG165250.019.3714711
Produced by FastQC (version 0.11.5)