FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5596341
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA26550.055.608722
CGAGTAG25800.054.416273
AGTAGCT101250.050.486725
GAGTAGC61600.050.1407054
GTAGCTG104150.048.2514086
GCTGGGA201850.046.8684359
CCCGAGT34800.045.371231
TAGCTGG117750.044.635037
TTGGGAG146650.043.9394726
TACTCGG27100.039.5850143
GGAGGCT155000.039.203489
CGGAGGT29850.038.8325047
GGGAGGC198950.038.2873738
GCACTTT130500.037.1256831
CTACTCG26500.036.6894762
TCGGGAG32050.036.1669356
GGAGGTT79350.036.1118748
GCTACTC63300.035.991431
TTTGGGA151250.035.891355
CTCGGGA30600.035.527895
Produced by FastQC (version 0.11.5)