FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5623429
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24450.058.61632
CGAGTAG25050.055.7594453
AGTAGCT99400.054.324955
GAGTAGC63350.051.029844
GTAGCTG104850.051.020516
TTGGGAG148650.048.5803346
GCTGGGA202950.046.7929279
CCCGAGT34600.046.4669841
TAGCTGG124550.044.858267
GGAGGCT159750.040.833059
GGGAGGC206850.040.6547978
TACTCGG28950.040.538113
CTACTCG29000.039.4860152
GCACTTT133750.038.703151
TGGGAGG201250.038.673577
CGGAGGT33650.038.0854767
TTTGGGA156450.037.920685
GGAGGTT82350.036.5457738
AAGTAGC57800.036.1238984
ACTTTGG144550.035.9619753
Produced by FastQC (version 0.11.5)