FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5335840
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22800.054.6289563
AGTAGCT93300.053.8623665
CCGAGTA23850.053.447922
GAGTAGC57600.052.872464
GTAGCTG98400.049.461046
CCCGAGT29150.048.4711071
GCTGGGA185050.047.4270679
TAGCTGG110400.045.2586947
TTGGGAG138150.044.975046
CGGAGGT28400.044.8710827
GGAGGCT138850.041.5334749
GGGAGGC180700.039.8829778
TACTCGG26000.039.321193
GGAGGTT80800.037.4239468
GCACTTT129300.036.796871
TGGGAGG182750.036.638467
CTACTCG26500.036.417062
TTTGGGA145250.036.0850145
CTCGGGA28650.035.4328545
AGGCGGA44500.035.1084254
Produced by FastQC (version 0.11.5)