FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4519285
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT120800.081.294465
CGAGTAG32900.080.5920643
GAGTAGC79700.078.6929554
GTAGCTG126700.077.111086
CCGAGTA34250.076.36362
GCTGGGA257500.064.895039
TAGCTGG155850.064.397577
TTGGGAG182350.061.277286
CCCGAGT45150.059.3816071
TGAGTAG60300.057.8320273
CAAGTAG53250.057.505543
CCAAGTA56150.057.4868662
AAGTAGC55900.057.4511874
GCACTTT149100.055.690131
TTTGGGA167750.055.6232035
CTGAGTA62600.055.4770132
ACTTTGG153750.053.5638123
AAGTGCT134300.052.5367975
GGAGGCT195400.052.3579989
CACTTTG158800.051.0437282
Produced by FastQC (version 0.11.5)