FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4519285
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT123850.083.4777765
CGAGTAG33250.082.931513
CCGAGTA34850.082.651622
GAGTAGC79050.080.96784
GTAGCTG133000.077.897166
GCTGGGA271450.067.288859
TTGGGAG181800.067.125626
TAGCTGG164050.065.523347
CCCGAGT45850.064.624431
CAAGTAG55950.063.1820643
GCACTTT148250.062.5374951
CCAAGTA60050.061.8772242
TTTGGGA169900.060.5128025
AAGTAGC60850.058.6858674
ACTTTGG157250.058.513043
CACTTTG159400.057.9605452
GGAGGCT202750.057.952449
AAGTGCT140300.057.4228675
TGAGTAG60300.055.7586173
CTGAGTA63000.053.8362122
Produced by FastQC (version 0.11.5)