FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573992
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24200.066.061512
CGAGTAG23550.063.894953
GAGTAGC60100.062.53274
AGTAGCT94000.061.8863225
GTAGCTG98050.059.10986
GCTGGGA197550.053.6465649
CCCGAGT32600.053.5189441
TTGGGAG141500.053.0178876
TAGCTGG117500.050.3056147
TACTCGG27650.047.390083
CTACTCG27150.047.212942
GCACTTT120200.045.22481
TCGGGAG32450.045.039396
GGAGGCT155100.044.9339649
GGGAGGC203100.043.38938
TTTGGGA143100.043.0167545
TGAGTAG52350.042.634013
CGGAGGT31050.041.9689567
CTCGGGA31900.041.527745
CTGAGTA54850.041.487892
Produced by FastQC (version 0.11.5)