FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192664
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26900.063.2054983
CCGAGTA28150.062.4462782
AGTAGCT107350.059.5560385
GAGTAGC67400.059.5105254
GTAGCTG112600.055.820116
CCCGAGT37100.050.623371
GCTGGGA220050.050.0265969
TTGGGAG154300.048.7135126
TAGCTGG132300.048.7055057
CGGAGGT36700.043.1590087
GGAGGTT86150.043.0394638
GGAGGCT163500.042.8900039
CAAGTAG52850.042.6672363
CCAAGTA57000.040.824682
TTTGGGA155300.040.518645
GGGAGGC215500.040.1579978
GCACTTT139950.040.105111
CTACTCG29700.038.568772
TACTCGG31100.038.4541173
AAGTGCT131400.038.4093975
Produced by FastQC (version 0.11.5)