FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192664
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27750.074.462183
CCGAGTA30300.068.9252
AGTAGCT108600.064.970035
GAGTAGC70150.064.453844
GTAGCTG116600.060.4506456
CCCGAGT39150.057.8090551
GCTGGGA230450.056.3294879
TTGGGAG162800.054.5729686
TAGCTGG135850.052.9447177
GCACTTT141800.047.6278881
GGAGGCT172800.046.5816549
TTTGGGA158700.046.4557885
GGGAGGC232900.045.0099648
CACTTTG151200.044.2947772
ACTTTGG150050.043.8557973
AAGTGCT139450.043.5753365
CTACTCG31450.043.506522
CAAGTAG51650.043.491173
TACTCGG32250.042.6403243
CGGAGGT38900.042.3840757
Produced by FastQC (version 0.11.5)