FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5621281
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA27750.063.5810242
CGAGTAG27750.060.4517943
GAGTAGC66150.054.9639324
AGTAGCT104150.053.7128225
GTAGCTG108800.051.6818926
CCCGAGT36550.051.4803471
GCTGGGA212200.049.9773949
TTGGGAG153350.048.0295036
TAGCTGG127150.045.299137
GGAGGCT163750.041.8573659
CGGAGGT36300.041.2546547
GGGAGGC214350.041.246888
TACTCGG27300.040.6138653
CTACTCG27100.040.392312
TCGGGAG33600.039.4270946
GCACTTT139450.038.979941
TGGGAGG206500.038.6658637
GGAGGTT89150.037.7955558
TTTGGGA161800.037.422585
AAGTGCT138150.035.8032235
Produced by FastQC (version 0.11.5)