FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567381
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28650.061.616513
AGTAGCT113750.059.81265
CCGAGTA30450.058.684042
GAGTAGC70850.057.7306674
GTAGCTG118550.056.6620646
GCTGGGA229650.050.4744649
TAGCTGG137600.050.439517
CCCGAGT38000.049.063921
TTGGGAG165550.048.4906466
GGAGGCT176300.042.3485689
CGGAGGT35400.042.099737
GCACTTT148500.040.9743231
TTTGGGA169250.040.7520145
TACTCGG31450.040.5516243
CTACTCG32250.040.2159582
CAAGTAG60000.039.9897423
GGGAGGC232800.039.709498
GGAGGTT89500.039.0953838
AAGTAGC62450.038.8593524
CCAAGTA64650.038.785232
Produced by FastQC (version 0.11.5)