FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5903992
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21800.051.5365072
CGAGTAG22200.048.971773
AGTAGCT88650.044.100445
GAGTAGC55800.042.9666864
CCCGAGT29700.042.4751
GTAGCTG90850.041.606026
GCTGGGA173900.040.5326429
TACTCGG29000.040.21973
CTACTCG29300.039.0819662
TTGGGAG134350.038.2632946
CGGAGGT28000.037.5419357
TAGCTGG104000.037.5221527
GGAGGCT143200.037.205799
TCGGGAG32650.036.16456
GCTACTC66650.035.583411
GGGAGGC176400.035.509338
CTCGGGA29000.035.5025755
GGAGGTT76250.033.331648
AGGCGGA41950.031.9229324
ACTCGGG36950.031.761064
Produced by FastQC (version 0.11.5)