FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5269184
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25700.064.1521153
AGTAGCT107750.062.0067945
GAGTAGC69050.060.9959144
CCGAGTA27250.059.988912
GTAGCTG112400.058.8010376
GCTGGGA224700.054.661889
TAGCTGG130000.053.1109247
TTGGGAG161700.052.9395756
CCCGAGT36200.050.981571
CTACTCG29400.050.948062
TACTCGG29500.050.7633063
GGAGGCT172800.048.2056779
GCACTTT137200.046.396841
TCGGGAG39150.045.6066866
GCTACTC74050.044.977851
GGGAGGC228650.044.3658038
TTTGGGA160300.043.7455675
CTCGGGA36350.043.573945
CGGAGGT37400.043.3131267
AAGTGCT135400.042.431945
Produced by FastQC (version 0.11.5)