FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5764300
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23200.048.4205132
CGAGTAG22200.046.700273
AGTAGCT87900.043.9840625
GAGTAGC54300.043.887224
CTACTCG21350.041.823132
GTAGCTG93100.041.218056
TTGGGAG130650.040.392856
GCTGGGA171800.040.272649
TACTCGG22300.040.033793
TAGCTGG102350.039.3218427
CCCGAGT31650.039.179961
CGGAGGT26800.038.684557
GGGAGGC169800.037.058148
GGAGGCT140300.035.7672049
CTCGGGA25500.035.574625
TCGGGAG28800.035.4981546
GCTACTC54900.033.8810351
GGAGGTT73650.033.5298238
TGGGAGG174100.032.793247
AGGCGGA41000.030.9057064
Produced by FastQC (version 0.11.5)