FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3960736
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24950.067.581383
CCGAGTA26500.066.619592
AGTAGCT92750.060.3144535
GAGTAGC60250.059.879374
GTAGCTG97350.056.4290666
CCCGAGT33850.054.005931
GCTGGGA193250.050.5934839
TTGGGAG136850.050.5583466
TAGCTGG115500.049.5563627
TACTCGG30300.049.4655083
CTACTCG29000.049.1981772
TCGGGAG34450.047.6496736
GGAGGCT156000.047.4441649
GCACTTT111700.045.21721
TTTGGGA128650.044.267095
GCTACTC68000.043.885471
CTCGGGA35050.043.7528045
GGGAGGC205950.043.558268
ACTTTGG117450.042.9464073
CACTTTG122450.041.4870072
Produced by FastQC (version 0.11.5)