FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5867022
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG29850.064.441793
CCGAGTA31450.061.1633452
AGTAGCT119900.060.5280765
GAGTAGC74500.059.1530724
GTAGCTG124800.057.5169836
TAGCTGG145250.050.75747
CCCGAGT42100.049.9222681
GCTGGGA240300.049.9156579
TTGGGAG177300.047.5920526
CGGAGGT38600.044.578577
GGAGGCT185700.041.4071049
CTACTCG32150.041.232292
CAAGTAG60900.041.1682623
TTTGGGA176100.040.3527685
TACTCGG32850.040.1343573
AAGTAGC65150.040.1212434
GGAGGTT93250.039.916948
GGGAGGC249300.039.6807638
TCGGGAG39500.039.3706176
GCACTTT153200.039.365081
Produced by FastQC (version 0.11.5)