FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5867022
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA31550.065.50932
CGAGTAG30600.064.937713
AGTAGCT120250.063.0458265
GAGTAGC75150.061.7936484
GTAGCTG126450.058.7589386
GCTGGGA250900.053.74659
TTGGGAG181850.053.527446
CCCGAGT41350.052.4727251
TAGCTGG152050.051.0914347
TACTCGG35300.047.929463
GGAGGCT194950.047.160759
CTACTCG36050.045.745742
GCACTTT158700.044.0138661
TTTGGGA178350.044.0013965
CAAGTAG59600.043.3668143
GGGAGGC258450.043.0114258
TCGGGAG44050.042.8221476
TGGGAGG249800.041.676287
CTCGGGA42550.041.4552545
CGGAGGT41100.041.3412177
Produced by FastQC (version 0.11.5)