FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5971384
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24900.048.3239172
AGTAGCT100600.048.164545
GAGTAGC63350.047.4021384
CGAGTAG24150.047.13953
GTAGCTG106450.045.585276
GCTGGGA189250.043.4831169
TTGGGAG148200.042.6536946
CGGAGGT27900.042.0623667
TAGCTGG120400.040.9015667
GGAGGCT154100.040.553419
CCCGAGT33600.040.008541
GGGAGGC199950.037.69958
GGAGGTT77250.035.8816648
TCGCGTT2700.034.6648985
TACTCGG27850.034.4089553
TGGGAGG197900.034.1245847
TCGGGAG32700.033.6863566
TTTGGGA157050.033.511185
CTACTCG27850.033.37412
GCACTTT143450.032.2950941
Produced by FastQC (version 0.11.5)