FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5971384
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA26800.061.2695432
CGAGTAG26500.059.50083
GAGTAGC66000.051.599064
AGTAGCT98350.051.0982065
GTAGCTG101200.049.374596
TTGGGAG157950.047.133046
GCTGGGA200050.046.787459
CCCGAGT39600.046.244091
CGGAGGT32100.044.186117
TAGCTGG119600.044.1262867
GGAGGCT161650.041.689319
TACTCGG26100.041.3785553
CTACTCG26900.040.9622462
GGGAGGC205150.040.640748
TTTGGGA153350.038.8281145
GCACTTT140600.037.843281
CTCGGGA30050.037.377025
TGGGAGG211450.037.182587
GGAGGTT87250.037.051478
GCTACTC67450.035.9149321
Produced by FastQC (version 0.11.5)