FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5779673
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC63400.057.2353254
CGAGTAG26100.055.1711923
AGTAGCT103550.054.8592345
CCGAGTA27900.051.883732
GTAGCTG111300.050.004286
TTGGGAG157900.049.2908636
GCTGGGA213550.048.0776069
CGGAGGT36500.047.7359287
CCCGAGT35900.046.1942521
TAGCTGG128100.044.6266447
GGGAGGC217150.041.7434928
GGAGGTT91050.040.96128
GCACTTT143500.040.8501051
GGAGGCT167000.040.0949179
TTTGGGA165100.039.7277685
TACTCGG28600.039.271863
AGGCGGA51750.039.2339134
TGGGAGG213400.039.2043847
ACTTTGG151100.038.357853
CTACTCG28500.036.8932882
Produced by FastQC (version 0.11.5)