FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5325047
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26000.056.808053
AGTAGCT105750.055.963215
GAGTAGC64900.055.1447874
CCGAGTA27100.055.0339162
GTAGCTG108500.054.8102156
TTGGGAG155850.050.3998346
GCTGGGA215800.049.9104279
TAGCTGG126550.047.7890437
CCCGAGT36050.045.702741
GGAGGCT167250.044.1233949
CTACTCG28750.042.6030272
GGGAGGC212700.041.769468
TACTCGG29500.041.7641333
GCACTTT140350.040.263081
TTTGGGA156600.039.9980355
TGGGAGG210350.038.710727
GCTACTC73650.038.657711
CGGAGGT33550.037.9832347
CAAGTAG53950.037.9276773
CCAAGTA58000.037.0184022
Produced by FastQC (version 0.11.5)