FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6764469
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28900.058.8326343
CCGAGTA31900.056.687482
CCCGAGT40550.048.1074451
GAGTAGC73000.047.2513774
AGTAGCT113300.047.0233655
GTAGCTG117100.045.55896
TTGGGAG168550.043.0142756
GCTGGGA222850.042.419359
TAGCTGG131250.041.7991647
GGAGGCT166200.040.590139
CGGAGGT33500.040.4040537
GGGAGGC213750.037.8255738
CTACTCG28950.036.8313182
GGAGGTT88950.036.099478
TACTCGG30500.035.6682053
TGGGAGG220000.035.3765037
TCGCGTT2650.035.319085
GCTACTC77950.033.08961
TTTGGGA180500.032.66775
GGCGGAG51900.032.599615
Produced by FastQC (version 0.11.5)