FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6764469
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC71600.055.8073164
CGAGTAG27350.055.017413
CCGAGTA29550.053.126712
AGTAGCT114050.051.7011035
GTAGCTG123800.047.629336
CCCGAGT37250.046.2579841
GCTGGGA226500.045.5500839
CGGAGGT38950.045.2866067
TTGGGAG168400.045.275696
TAGCTGG138200.043.3437467
GGAGGCT181500.041.8888559
CTACTCG32200.041.8215032
TACTCGG33500.041.0487333
GGAGGTT98050.038.5498668
GGGAGGC232200.038.4477238
CTCGGGA38300.038.347995
TCGGGAG41100.038.187916
GCTACTC78850.037.0312541
AGGCGGA57400.036.876244
TGGGAGG227300.034.9688077
Produced by FastQC (version 0.11.5)