FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5419201
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22400.050.5004652
TCGCGTT2400.047.997635
CGAGTAG21450.047.02673
AGTAGCT83850.040.6992455
CTCGCGT3800.039.7985274
CCCGAGT32650.039.6170961
GCTGGGA161050.039.2504659
TTGGGAG122850.039.2068566
GAGTAGC52300.038.693314
GTAGCTG87550.038.156886
CGGAGGT25700.036.9787187
TAGCTGG98550.035.9434287
GGAGGCT135700.034.6453069
CTACTCG22450.034.3408622
GGGAGGC165800.033.957318
CGCGTTG3450.033.3896566
TACTCGG24300.032.615963
TGGGAGG162700.032.1705067
GGAGGTT66900.032.0701648
GCTACTC57350.030.7446251
Produced by FastQC (version 0.11.5)