FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4405073
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT99350.072.5404365
CCGAGTA28300.070.7827452
CGAGTAG27100.070.726373
GTAGCTG104850.067.63666
GAGTAGC64950.067.629884
TAGCTGG124150.058.455837
CCCGAGT36000.058.203651
GCTGGGA208800.057.514799
TTGGGAG148450.055.3374866
CAAGTAG46900.049.9320683
AAGTAGC50200.049.7752764
CCAAGTA50100.049.475452
GGAGGCT164450.048.2898189
TGAGTAG52600.045.8910643
TTTGGGA147400.045.4743165
GCACTTT125200.045.4176981
CTGAGTA54950.045.2397732
CCCAAGT62200.044.7223931
GGGAGGC214650.044.6102568
TGGGAGG205400.042.8686687
Produced by FastQC (version 0.11.5)