FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4405073
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28350.072.378823
CCGAGTA29650.071.412712
AGTAGCT105500.071.383685
GAGTAGC66500.070.482144
GTAGCTG111000.066.614266
TTGGGAG158800.061.887356
GCTGGGA216950.061.6603249
CCCGAGT36200.059.349141
TAGCTGG129650.058.198067
GCACTTT124050.055.9094281
TTTGGGA144650.055.448085
GGAGGCT168000.053.4412849
CAAGTAG47150.052.986783
ACTTTGG132300.052.9508253
CACTTTG137250.050.951822
GGGAGGC223350.050.932028
CTTTGGG151800.049.5637634
AAGTGCT123800.049.4912765
CCAAGTA52550.048.9269682
TCGGGAG36900.048.583946
Produced by FastQC (version 0.11.5)