FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4391544
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23750.063.402132
CGAGTAG22750.063.02213
AGTAGCT89750.056.71525
GAGTAGC57450.054.1479954
GTAGCTG93400.053.496716
CCCGAGT30900.052.15021
GCTGGGA172850.050.441659
CGGAGGT27400.047.8228387
TAGCTGG111000.047.4791157
TTGGGAG127550.046.1729166
GGAGGTT67400.042.0872768
CTACTCG22300.040.7086642
GGAGGCT130100.040.398029
CAAGTAG44850.039.3572583
AAGTAGC46750.038.8157164
TTTGGGA130600.038.6446465
CCAAGTA50700.038.368692
GGGAGGC176250.038.316718
GCTACTC58700.038.2852861
GCACTTT116100.038.1538051
Produced by FastQC (version 0.11.5)