FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4391544
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22250.061.1571923
AGTAGCT87300.058.7193875
CCGAGTA23900.058.4545752
GAGTAGC54100.057.6243974
GTAGCTG92900.054.4822966
TTGGGAG131750.052.2970436
CGGAGGT28800.051.4980167
GCTGGGA183400.050.5236859
CCCGAGT30900.047.3552251
TAGCTGG109400.047.1206747
GCACTTT116500.045.2913741
GGAGGCT141900.044.953879
GGAGGTT72450.043.6256528
TTTGGGA135150.043.5232965
GGGAGGC184150.043.0458568
ACTTTGG123100.042.2274553
CTACTCG25650.041.8324782
CACTTTG128200.040.7253342
TGGGAGG179800.040.5234687
GCTACTC61900.040.2917181
Produced by FastQC (version 0.11.5)