FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6369385
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA26950.026.724372
AGTAGCT103650.026.6039225
GCTGGGA208900.026.0210449
CGAGTAG26850.025.7420523
GTAGCTG110350.025.0538866
GAGTAGC64450.025.0231134
TAGCTGG118000.024.4668797
TACTCGG29000.022.343993
TTGGGAG142450.022.1880056
CCCGAGT40200.022.0723421
CTACTCG29100.020.5424082
CCCAAAG167850.020.1567671
GCTACTC67500.019.9851421
AAGTGCT134000.019.933585
AAAGTGC134950.019.6865524
GGAGGCT163900.019.415999
CCAAAGT151850.018.7822072
GTGCTGG139400.018.6965737
CAAGTAG58250.018.663683
GGGAGGC198400.018.471088
Produced by FastQC (version 0.11.5)