FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643232
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT23450.039.606227
CGAGTAG19100.039.2027473
CCGAGTA20600.039.155992
TTGGGAG113700.035.9670146
GAGTAGC48250.035.513724
GGAGGCT125300.035.1655129
AGTAGCT77950.034.9134065
AGGCGGA32250.034.3801354
GCTGGGA155350.034.3418359
GGGAGGC148150.033.094978
CTACTCG20100.032.9639432
CCCGAGT28900.032.6896441
TAGCTGG89350.032.5539257
TACTCGG21250.032.5258643
GTAGCTG84050.032.2082186
GGAGGTT65950.031.8775238
TCGGGAG26550.031.1852956
GCTACTC52650.030.1342951
CTCGGGA24800.029.9021285
GGCGGAG36050.029.7575765
Produced by FastQC (version 0.11.5)