FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083931
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT86200.063.3938335
CGAGTAG21850.061.2877963
CCGAGTA22550.060.6831972
GAGTAGC54850.059.067474
GTAGCTG93000.058.6811686
GCTGGGA186250.054.9300889
TTGGGAG129600.053.4975366
TAGCTGG104900.053.1910677
CCCGAGT28500.049.8417971
GGAGGCT142100.047.018249
CTACTCG24350.046.436782
AAGTGCT109500.046.354035
TTTGGGA124700.046.0732545
TACTCGG25200.045.4264763
GCACTTT110750.044.7286031
CAAGTAG42050.044.1739463
ACTTTGG112750.043.485363
GGGAGGC184800.043.4785168
GCTACTC60700.043.3587271
AAGTAGC45300.042.5940634
Produced by FastQC (version 0.11.5)