FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083931
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22450.074.4042363
CCGAGTA23300.071.689922
GAGTAGC53350.067.612864
AGTAGCT86750.066.231535
GTAGCTG93000.061.0062946
CCCGAGT30300.058.4560051
GCTGGGA186600.057.684739
TTGGGAG139500.057.2385566
TAGCTGG109500.051.8793147
GCACTTT113650.050.9358181
TTTGGGA132700.049.9169735
GGAGGCT153150.049.4572649
CTACTCG25700.049.026512
TACTCGG26050.048.0914153
ACTTTGG119950.048.0793153
AAGTGCT109750.048.0217445
CACTTTG122150.046.6239472
GCTACTC62550.045.92851
TCGGGAG32050.045.828276
GGGAGGC204850.045.4458168
Produced by FastQC (version 0.11.5)