FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5203784
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18300.048.4071462
CGAGTAG18050.045.4725763
CGGAGGT22500.043.8389327
TCGCGTT2050.042.1453175
AGTAGCT71950.040.8273625
GAGTAGC45050.038.995724
GTAGCTG77400.038.324656
GCTGGGA145200.038.180899
CCCGAGT26450.037.3516731
TAGCTGG82200.036.9626057
TTGGGAG108700.036.8933036
TACTCGG19900.035.094623
GGAGGCT117250.033.6503569
GGAGGTT63950.033.437818
GGGAGGC139800.033.1150748
CTACTCG20100.032.9645542
AGGCGGA32650.031.3131754
TGGGAGG144350.029.976417
GCTACTC49400.029.9255451
GAGGTTG81400.029.4538299
Produced by FastQC (version 0.11.5)