FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5203784
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18500.053.707042
CGAGTAG18800.049.0202983
CGGAGGT23050.046.5418177
GAGTAGC46850.046.257354
TACTCGG19850.043.8882943
CCCGAGT25800.043.8147661
AGTAGCT78600.042.2285735
TTGGGAG119100.042.1355866
GCTGGGA149850.041.417399
CTACTCG20000.040.6791882
GGGAGGC149250.038.5925648
CTCGGGA23950.038.479775
GGAGGCT121450.038.2379349
GTAGCTG85100.038.072466
TCGGGAG25300.036.7110866
GCTACTC47000.036.613441
GGAGGTT68100.035.9467668
TGGGAGG150650.035.366387
TAGCTGG93900.035.3478787
AGGCGGA35950.034.647434
Produced by FastQC (version 0.11.5)