FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161552
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15000.051.3586163
CCGAGTA16500.046.2677542
GAGTAGC38500.045.8169444
AGTAGCT59650.044.1765145
TTGGGAG95400.043.7724046
GCTGGGA124150.041.2908749
TACTCGG16900.040.0462573
GTAGCTG66400.039.7941066
CGGAGGT22100.039.7455377
GGAGGCT102900.039.6723989
TAGCTGG71400.039.5283477
CTACTCG16800.039.0111352
GGGAGGC128050.038.290268
CCCGAGT22100.037.5381161
CTCGGGA18650.037.0606655
GGAGGTT55150.036.2927678
TCGGGAG20900.034.793326
TTTGGGA102800.034.6683275
GCTACTC39500.034.3342631
AGGCGGA30450.034.0483444
Produced by FastQC (version 0.11.5)